KMID : 1225720210130060882
|
|
Allergy, Asthma & Immunology Research : AAIR 2021 Volume.13 No. 6 p.882 ~ p.895
|
|
Activation of Dopamine D2 Receptor Alleviates Neuroinflammation in a Mouse Model of Allergic Rhinitis With Olfactory Dysfunction
|
|
Liu Peiqiang
Qin Danxue Lv Hao Fan Wenjun Zhou Fangwei Gao Ziang Tao Zezhang Xu Yu
|
|
Abstract
|
|
|
Purpose: Allergic rhinitis (AR) is a common otolaryngology disease and one of the clinical causes of olfactory dysfunction (OD). The olfactory bulb serves as a transfer station for olfactory information transmission, and alleviating its neuroinflammation may be expected to improve AR-induced OD. Recent studies have suggested that the dopamine D2 receptor acts as a key target in regulating immune functions and neuroinflammatory reaction. However, the effect of dopamine D2 receptor on AR-induced neuroinflammation is still unknown.
Methods: An AR mouse model with OD induced by ovalbumin were constructed. The buried food pellet test was to evaluate the olfactory function of the mice. Immunofluorescence staining, hematoxylin and eosin staining, enzyme-linked immunosorbent assay and western blotting were also used to investigate the molecular mechanisms underlying the anti-inflammatory effects of the dopamine D2 receptor in AR-induced OD.
Results: We found that AR-induced OD has a relationship with inflammatory responses in the olfactory bulb. Nasal administration of quinpirole (Quin, a dopamine D2 receptor agonist, 3 mg/kg) improved olfactory function in mice, inhibited the expression of toll-like receptor 4 (TLR4)/nuclear factor-¥êB (NF-¥êB) signalings and the levels of tumor necrosis factor-¥á, interleukin (IL)-1¥â and IL-6 in the olfactory bulb. In vitro, Quin (20 ¥ìmol/L) inhibited the release of TLR4/NF-¥êB signalings-dependent inflammatory cytokines in cultured microglia.
Conclusions: Activation of the dopamine D2 receptor inhibits the release of inflammatory cytokines through TLR4/NF-¥êB signaling in the olfactory bulb microglia, and protects olfactory function.
|
|
KEYWORD
|
|
Allergic rhinitis, dopamine D2 receptor, microglia, inflammation
|
|
FullTexts / Linksout information
|
|
|
|
Listed journal information
|
|
|
|